Document Type : Original Article

Authors

1 Assistant Professor of Exercise physiology, Sport Science Department, Human Faculty, Semnan University, Semnan, Iran

2 M.Sc of Exercise physiology, Sport Science Department, Human Faculty, Semnan University, Semnan, Iran

Abstract

Introduction: The aim of this study was to evaluate the acute effect of probiotic supplementation on plasma fibrinogen levels of young soccer players following an aerobic and anaerobic exercise.
Method: Twenty-eight soccer players (Mean of age 20.68±2.10 yrs., Height 1.73 ± 0.14 m, Weight 64.49±7.39 kg and Vo2max 59.78±5.30 ml/kg/min) were selected and randomly assigned into aerobic (n=14) and anaerobic (n=14) groups. Huff and RAST tests were used for aerobic and anaerobic training protocols, respectively. Probiotic (910 units/g) and placebo capsules were used as  double-blind method and counterbalance style in two stages (48 hours after the initial blood sampling and two weeks after the initial tests). Blood samples were taken in three stages before the supplementation, immediately after the tests, and in the last stage after two weeks immediately after the Huff & RAST tests. Data were analyzed by two-way mixed design or repeated measure for in-group and inter-group analysis at a significance level of P <0.05.
Result: Data analysis showed that aerobic and anaerobic exercise combined with probiotic supplementation had a non-significant effects on fibrinogen reduction in aerobic test (p ≥0.296) and in anaerobic test (p ≥0.879).
Conclusion: According to the findings of this study, it can be concluded that probiotic supplementation causes non-significant reduction of fibrinogen in response to an aerobic and anaerobic exercise session in soccer players. Besides, it is not likely to contribute to the rheology of blood and the reduction of blood clots in soccer players.

Keywords

A, Rashidlamir, A, Hashemi Javaheri, & M, Jaafari. (2011). The effect of regular aerobic training with weight loss on concentrations of fibrinogen and resistin in healthy and overweight men. Tehran University Medical Journal, 68(12), 710-717.
Agerholm-Larsen, L., Raben, A., Haulrik, N., Hansen, A. S., Manders, M., & Astrup, A. (2000). Effect of 8 week intake of probiotic milk products on risk factors for cardiovascular diseases. Eur J Clin Nutr, 54(4), 288-297. doi: 10.1038/sj.ejcn.1600937
Ahmadizad, S., & El-Sayed, M. S. (2005). The acute effects of resistance exercise on the main determinants of blood rheology. J Sports Sci, 23(3), 243-249. doi: 10.1080/02640410410001730151
Ali, A. A., Velasquez, M. T., Hansen, C. T., Mohamed, A. I., & Bhathena, S. J. (2004). Effects of soybean isoflavones, probiotics, and their interactions on lipid metabolism and endocrine system in an animal model of obesity and diabetes. J Nutr Biochem, 15(10), 583-590. doi: 10.1016/j.jnutbio.2004.04.005
Alvarez, S., Herrero, C., Bru, E., & Perdigon, G. (2001). Effect of Lactobacillus casei and yogurt administration on prevention of Pseudomonas aeruginosa infection in young mice. J Food Prot, 64(11), 1768-1774. doi: 10.4315/0362-028x-64.11.1768
Bangsbo, J., Nørregaard, L., & Thorsø, F. (1991). Activity profile of competition soccer. Can J Sport Sci, 16(2), 110-116.
BIZHEH, N., & JAAFARI, M. (2012). EFFECTS OF REGULAR AEROBIC EXERCISE ON CARDIORESPIRATORY FITNESS AND LEVELS OF FIBRINOGEN, FIBRIN D-DIMER AND URIC ACID IN HEALTHY AND INACTIVE MIDDLE AGED MEN. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 14(3), -.
Casas, J. P., Shah, T., Hingorani, A. D., Danesh, J., & Pepys, M. B. (2008). C-reactive protein and coronary heart disease: a critical review. J Intern Med, 264(4), 295-314. doi: 10.1111/j.1365-2796.2008.02015.x
Cheitlin, M. D., De Castro, C. M., & McAllister, H. A. (1974). Sudden death as a complication of anomalous left coronary origin from the anterior sinus of Valsalva, A not-so-minor congenital anomaly. Circulation, 50(4), 780-787. doi: 10.1161/01.cir.50.4.780
Cicek, H., Bayil, S., Zer, Y., Celik, A., & Geyikli, I. (2007). Comparison of Lipoprotein(a) levels between elderly and middle-aged men with coronary artery disease. Ann N Y Acad Sci, 1100, 179-184. doi: 10.1196/annals.1395.016
Corrado, D., Basso, C., Rizzoli, G., Schiavon, M., & Thiene, G. (2003). Does sports activity enhance the risk of sudden death in adolescents and young adults? J Am Coll Cardiol, 42(11), 1959-1963. doi: 10.1016/j.jacc.2003.03.002
DABIDI ROUSHAN, V.A., GAEINI, A.A., RAVASI, A.A., & JAVADI, EBRAHIM. (2005). THE EFFECT OF THE CONTINUOUS TRAINING OF CRP OF STRAIN WISTAR 14848 RATS. OLYMPIC, 13(2 (SERIAL 30)), -.
Diamond, G. A., & Forrester, J. S. (1979). Analysis of probability as an aid in the clinical diagnosis of coronary-artery disease. N Engl J Med, 300(24), 1350-1358. doi: 10.1056/nejm197906143002402
Eriksson-Berg, Margita, Egberg, Nils, Eksborg, Staffan, & Schenck-Gustafsson, Karin. (2002). Retained fibrinolytic response and no coagulation activation after acute physical exercise in middle-aged women with previous myocardial infarction. Thrombosis research, 105(6), 481-486.
Furukawa, F., Kazuma, K., Kojima, M., & Kusukawa, R. (2008). Effects of an off-site walking program on fibrinogen and exercise energy expenditure in women. Asian Nurs Res (Korean Soc Nurs Sci), 2(1), 35-45. doi: 10.1016/s1976-1317(08)60027-4
Geffken, D. F., Cushman, M., Burke, G. L., Polak, J. F., Sakkinen, P. A., & Tracy, R. P. (2001). Association between physical activity and markers of inflammation in a healthy elderly population. Am J Epidemiol, 153(3), 242-250. doi: 10.1093/aje/153.3.242
Ghanbari-Niaki, Abbass, Behzad Khameslo, Mostafa, & Tayebi, Seyed Morteza. (2013). Effect of Pyramidal Training on Plasma Lipid Profile and Fibrinogen, and Blood Viscosity of Untrained Young Men. Annals of Applied Sport Science, 1(3), 47-56.
Hatakka, K., Martio, J., Korpela, M., Herranen, M., Poussa, T., Laasanen, T., . . . Korpela, R. (2003). Effects of probiotic therapy on the activity and activation of mild rheumatoid arthritis--a pilot study. Scand J Rheumatol, 32(4), 211-215. doi: 10.1080/03009740310003695
Hu, F. B., Meigs, J. B., Li, T. Y., Rifai, N., & Manson, J. E. (2004). Inflammatory markers and risk of developing type 2 diabetes in women. Diabetes, 53(3), 693-700. doi: 10.2337/diabetes.53.3.693
Hull, S. S., Jr., Vanoli, E., Adamson, P. B., Verrier, R. L., Foreman, R. D., & Schwartz, P. J. (1994). Exercise training confers anticipatory protection from sudden death during acute myocardial ischemia. Circulation, 89(2), 548-552. doi: 10.1161/01.cir.89.2.548
ICMR-DBT guidelines for evaluation of probiotics in food. (2011). Indian J Med Res, 134(1), 22-25.
Isasi, C. R., Starc, T. J., Tracy, R. P., Deckelbaum, R., Berglund, L., & Shea, S. (2000). Inverse association of physical fitness with plasma fibrinogen level in children: the Columbia University BioMarkers Study. Am J Epidemiol, 152(3), 212-218. doi: 10.1093/aje/152.3.212
Jahani Ghaeh Ghashlagh, Gholamreza, َAbcar, Alireza, & Haydari, Hossain. (2016). The Effect of High-Intensity Intermittent Training (HIIT) and Consumption of Probiotic Supplement on Immune Cells, C – reactive Protein, and IgA in Young Football Player. Qom Univ Med Sci J, 10(8), 36-46.
JAHANI QIEQESHLAQ, GHOLAM REZA, ABKAR, ALI REZA, & HEIDARI, HOOSSEIN. (2016). THE EFFECT OF HIGH-INTENSITY INTERMITTENT TRAINING (HIIT) AND CONSUMPTION OF PROBIOTIC SUPPLEMENT ON IMMUNE CELLS, C - REACTIVE PROTEIN, AND IGA IN YOUNG FOOTBALL PLAYER. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 10(8), -.
Kamath, S., & Lip, G. Y. (2003). Fibrinogen: biochemistry, epidemiology and determinants. QJM, 96(10), 711-729. doi: 10.1093/qjmed/hcg129
Maron, B. J., Epstein, S. E., & Roberts, W. C. (1986). Causes of sudden death in competitive athletes. J Am Coll Cardiol, 7(1), 204-214. doi: 10.1016/s0735-1097(86)80283-2
Maron, B. J., Nichols, P. F., 3rd, Pickle, L. W., Wesley, Y. E., & Mulvihill, J. J. (1984). Patterns of inheritance in hypertrophic cardiomyopathy: assessment by M-mode and two-dimensional echocardiography. Am J Cardiol, 53(8), 1087-1094. doi: 10.1016/0002-9149(84)90643-x
Maron, B. J., Shirani, J., Poliac, L. C., Mathenge, R., Roberts, W. C., & Mueller, F. O. (1996). Sudden death in young competitive athletes. Clinical, demographic, and pathological profiles. JAMA, 276(3), 199-204.
Mazidi, M., Rezaie, P., Kengne, A. P., Mobarhan, M. G., & Ferns, G. A. (2016). Gut microbiome and metabolic syndrome. Diabetes Metab Syndr, 10(2 Suppl 1), S150-157. doi: 10.1016/j.dsx.2016.01.024
Nocon, M., Hiemann, T., Müller-Riemenschneider, F., Thalau, F., Roll, S., & Willich, S. N. (2008). Association of physical activity with all-cause and cardiovascular mortality: a systematic review and meta-analysis. Eur J Cardiovasc Prev Rehabil, 15(3), 239-246. doi: 10.1097/HJR.0b013e3282f55e09
Parsian H, Zafari A. (2013). The effect of different types of exercise on plasma fibrinogen levels in non-athlete males. European Journal of Experimental Biology, 3(3), 246-230.
Parvaneh Nazar Ali, Parichehr Hanachi. (2011). To investigate the fibrinogen and some of coagulation factors in anaerobic exercise training women. World Applied Sciences Journal, 12(1).
Patelis, N., Karaolanis, G., Kouvelos, G. N., Hart, C., & Metheiken, S. (2016). The effect of exercise on coagulation and fibrinolysis factors in patients with peripheral arterial disease. Exp Biol Med (Maywood), 241(15), 1699-1707. doi: 10.1177/1535370216660215
Rana, J. S., Arsenault, B. J., Després, J. P., Côté, M., Talmud, P. J., Ninio, E., . . . Boekholdt, S. M. (2011). Inflammatory biomarkers, physical activity, waist circumference, and risk of future coronary heart disease in healthy men and women. Eur Heart J, 32(3), 336-344. doi: 10.1093/eurheartj/ehp010
Rankinen, T., Väisänen, S., Penttilä, I., & Rauramaa, R. (1995). Acute dynamic exercise increases fibrinolytic activity. Thromb Haemost, 73(2), 281-286.
Roberfroid, M. B. (2000). Prebiotics and probiotics: are they functional foods? Am J Clin Nutr, 71(6 Suppl), 1682S-1687S; discussion 1688S-1690S. doi: 10.1093/ajcn/71.6.1682S
Salarkia, Nahid, Ghadamli, Leili, Zaeri, Farid, & Sabaghian Rad, Leila. (2013). Effects of probiotic yogurt on performance, respiratory and digestive systems of young adult female endurance swimmers: a randomized controlled trial. Medical Journal of the Islamic Republic Of Iran, 27(3), 141-146.
Schrezenmeir, J., & de Vrese, M. (2001). Probiotics, prebiotics, and synbiotics--approaching a definition. Am J Clin Nutr, 73(2 Suppl), 361S-364S. doi: 10.1093/ajcn/73.2.361s
Shoaei, T., Heidari-Beni, M., Tehrani, H. G., Feizi, A., Esmaillzadeh, A., & Askari, G. (2015). Effects of Probiotic Supplementation on Pancreatic β-cell Function and C-reactive Protein in Women with Polycystic Ovary Syndrome: A Randomized Double-blind Placebo-controlled Clinical Trial. Int J Prev Med, 6, 27. doi: 10.4103/2008-7802.153866
Siscovick, D. S., Weiss, N. S., Fletcher, R. H., & Lasky, T. (1984). The incidence of primary cardiac arrest during vigorous exercise. N Engl J Med, 311(14), 874-877. doi: 10.1056/nejm198410043111402
Slimani, M., Znazen, H., Miarka, B., & Bragazzi, N. L. (2019). Maximum Oxygen Uptake of Male Soccer Players According to their Competitive Level, Playing Position and Age Group: Implication from a Network Meta-Analysis. J Hum Kinet, 66, 233-245. doi: 10.2478/hukin-2018-0060
Stratton, J. R., Chandler, W. L., Schwartz, R. S., Cerqueira, M. D., Levy, W. C., Kahn, S. E., . . . Abrass, I. B. (1991). Effects of physical conditioning on fibrinolytic variables and fibrinogen in young and old healthy adults. Circulation, 83(5), 1692-1697. doi: 10.1161/01.cir.83.5.1692
Taylor, A. G., Ignaszewski, A. I., Bredin, S. S. D., Hill, J. S., Shellington, E. M., & Warburton, D. E. R. (2021). High Intensity Interval Training Leads to Similar Inflammatory Activation as Seen With Traditional Training in Chronic Heart Failure. Front Cardiovasc Med, 8, 752531. doi: 10.3389/fcvm.2021.752531
Thompson, P. D., Franklin, B. A., Balady, G. J., Blair, S. N., Corrado, D., Estes, N. A., 3rd, . . . Costa, F. (2007). Exercise and acute cardiovascular events placing the risks into perspective: a scientific statement from the American Heart Association Council on Nutrition, Physical Activity, and Metabolism and the Council on Clinical Cardiology. Circulation, 115(17), 2358-2368. doi: 10.1161/circulationaha.107.181485